|
KMID : 0613820080180050639
|
|
Journal of Life Science
2008 Volume.18 No. 5 p.639 ~ p.645
|
|
|
Isolation of Bacteria Producing Cell Wall Degrading Enzyme and Characterization of CMCase.
|
|
Lee Yong-Seok
Park In-Hye
Zhou Yi
Kim Keun-Ki
Choi Si-Lim
Choi Yong-Lark
|
|
|
Abstract
|
|
|
|
A CMCase-producing bacterium, Klebsiella sp. Sc, was isolated from Busan area’s soil sample. This strain was identified on the basis of phylogenetic analysis of the 165 rDNA and rpoB (RNA polymerase B subunit) sequences. The gene encoding CMCase was cloned by genome library. The CMCase gene consisted of an open reading frame of 1,002 nucleotides and encodes 333 amino acids with a deduced molecular weight of 37,666 Da. The deduced amino acid sequence of the CMCase from Klebsiella sp. exhibits 95% identities and 97% positives to those from Cellulomonas uda and 58% identities and 72% positives to those from Erwinia chrysanthemi. The CMCase contain a conserved motif with the consensus sequence, ASDGDTLIAWALLRAQKQW. This result shows CMCase protein from Klebsiella sp. Sc belongs to glycoside hydrolase family 8, A-[ST]-D-[AG]-D-X(2)-[IM]-A-[SA]-[LIVM]-[LIVMG]-A-X(3)-[FW].
|
|
|
KEYWORD
|
|
|
Klebsiella sp., CMCase, glycosyl hydrolase family 8, endoglucanase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|